Acute alcohol consumption increases systemic endotoxin bioactivity for days in healthy volunteers-with reduced intestinal barrier loss in female.

Sturm, Ramona; Haag, Florian; Janicova, Andrea; Xu, Baolin; Vollrath, Jan Tilmann; Bundkirchen, Katrin; Dunay, Ildiko Rita; Neunaber, Claudia; Marzi, Ingo; Relja, Borna

doi:10.1007/s00068-021-01666-4

by Ramona Sturm, Florian Haag, Andrea Janicova, Baolin Xu, Jan Tilmann Vollrath, Katrin Bundkirchen, Ildiko Rita Dunay, Claudia Neunaber, Ingo Marzi, Borna Relja

Abstract:

OBJECTIVE: Trauma is the most common cause of death among young adults. Alcohol intoxication plays a significant role as a cause of accidents and as a potent immunomodulator of the post-traumatic response to tissue injury. Polytraumatized patients are frequently at risk to developing infectious complications, which may be aggravated by alcohol-induced immunosuppression. Systemic levels of integral proteins of the gastrointestinal tract such as syndecan-1 or intestinal fatty acid binding proteins (FABP-I) reflect the intestinal barrier function. The exact impact of acute alcohol intoxication on the barrier function and endotoxin bioactivity have not been clarified yet. METHODS: 22 healthy volunteers received a precisely defined amount of alcohol (whiskey-cola) every 20 min over a period of 4 h to reach the calculated blood alcohol concentration (BAC) of 1‰. Blood samples were taken before alcohol drinking as a control, and after 2, 4, 6, 24 and 48 h after beginning with alcohol consumption. In addition, urine samples were collected. Intestinal permeability was determined by serum and urine values of FABP-I, syndecan-1, and soluble (s)CD14 as a marker for the endotoxin translocation via the intestinal barrier by ELISA. BAC was determined. RESULTS: Systemic FABP-I was significantly reduced 2 h after the onset of alcohol drinking, and remained decreased after 4 h. However, at 6 h, FABP-I significantly elevated compared to previous measurements as well as to controls (p \textless 0.05). Systemic sCD14 was significantly elevated after 6, 24 and 48 h after the onset of alcohol consumption (p \textless 0.05). Systemic FABP-I at 2 h after drinking significantly correlated with the sCD14 concentration after 24 h indicating an enhanced systemic LPS bioactivity. Women showed significantly lower levels of syndecan-1 in serum and urine and urine for all time points until 6 h and lower

Reference:

Acute alcohol consumption increases systemic endotoxin bioactivity for days in healthy volunteers-with reduced intestinal barrier loss in female. (Ramona Sturm, Florian Haag, Andrea Janicova, Baolin Xu, Jan Tilmann Vollrath, Katrin Bundkirchen, Ildiko Rita Dunay, Claudia Neunaber, Ingo Marzi, Borna Relja), In European journal of trauma and emergency surgery : official publication of the European Trauma Society, volume 48, 2022.

Bibtex Entry:

@article{sturm_acute_2022,
	title = {Acute alcohol consumption increases systemic endotoxin bioactivity for days in  healthy volunteers-with reduced intestinal barrier loss in female.},
	volume = {48},
	copyright = {© 2021. The Author(s).},
	issn = {1863-9941 1863-9933 1863-9933},
	doi = {10.1007/s00068-021-01666-4},
	abstract = {OBJECTIVE: Trauma is the most common cause of death among young adults. Alcohol  intoxication plays a significant role as a cause of accidents and as a potent  immunomodulator of the post-traumatic response to tissue injury. Polytraumatized  patients are frequently at risk to developing infectious complications, which may  be aggravated by alcohol-induced immunosuppression. Systemic levels of integral  proteins of the gastrointestinal tract such as syndecan-1 or intestinal fatty  acid binding proteins (FABP-I) reflect the intestinal barrier function. The exact  impact of acute alcohol intoxication on the barrier function and endotoxin  bioactivity have not been clarified yet. METHODS: 22 healthy volunteers received  a precisely defined amount of alcohol (whiskey-cola) every 20 min over a period  of 4 h to reach the calculated blood alcohol concentration (BAC) of 1‰. Blood  samples were taken before alcohol drinking as a control, and after 2, 4, 6, 24  and 48 h after beginning with alcohol consumption. In addition, urine samples  were collected. Intestinal permeability was determined by serum and urine values  of FABP-I, syndecan-1, and soluble (s)CD14 as a marker for the endotoxin  translocation via the intestinal barrier by ELISA. BAC was determined. RESULTS:  Systemic FABP-I was significantly reduced 2 h after the onset of alcohol  drinking, and remained decreased after 4 h. However, at 6 h, FABP-I significantly  elevated compared to previous measurements as well as to controls (p {\textless} 0.05).  Systemic sCD14 was significantly elevated after 6, 24 and 48 h after the onset of  alcohol consumption (p {\textless} 0.05). Systemic FABP-I at 2 h after drinking  significantly correlated with the sCD14 concentration after 24 h indicating an  enhanced systemic LPS bioactivity. Women showed significantly lower levels of  syndecan-1 in serum and urine and urine for all time points until 6 h and lower},
	language = {eng},
	number = {3},
	journal = {European journal of trauma and emergency surgery : official publication of the  European Trauma Society},
	author = {Sturm, Ramona and Haag, Florian and Janicova, Andrea and Xu, Baolin and Vollrath, Jan Tilmann and Bundkirchen, Katrin and Dunay, Ildiko Rita and Neunaber, Claudia and Marzi, Ingo and Relja, Borna},
	month = jun,
	year = {2022},
	pmid = {33839799},
	pmcid = {PMC9192383},
	keywords = {*Alcoholic Intoxication, *Lipopolysaccharide Receptors, alcohol, Alcohol Drinking/adverse effects, Barrier, Biomarkers, Blood Alcohol Content, Endotoxins, FABP, Female, Gender, Healthy Volunteers, Humans, SCD14, Syndecan-1, Young Adult},
	pages = {1569--1577}
}